Air-Dryable RT-qPCR and qPCR mixes are glycerol-free mixes that contain optimized excipients compatible with air and oven drying. The mixes are easy to use and ideal for developing ambient temperature, multiplex molecular assays. The Air-Dryable RT-qPCR Mix exhibits high tolerance towards inhibitors and can also be used directly with crude samples such as sputum in Universal Transport Media. Advantages to ambient-temperature assays include room temperature shipping and storage, extended shelf-life and increased flexibility in sample volume.
Activity of Air-Dryable 1-Step RT-qPCR Mix in both wet and air-dried formats were compared on 10-fold dilution mouse RNA template. The air-dried mix showed no loss of activity and sensitivity when compared to freshly prepared wet mix up to the assay limit of detection.
Amplification profile of a mouse RNA target spiked into samples containing 10%, 5% or 0% artificial sputum in Universal Transport Media (UTM). The data illustrates that the performance of Air-Dryable 1-Step RT-qPCR Mix (MDX095) exhibits high tolerance towards inhibitors present in artificial sputum and UTM.
