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SensiFAST™ Lyo-Ready Mix - 5/21/2018
 

MAY 2018

 

 

SensiFAST™ Lyo-Ready Mix
Lyophilization-friendly qPCR Master Mix

Ideal for incorporation into high-throughput, diagnostic systems and point-of-care devices

SensiFAST™ Lyo-Ready Key Performance Characteristics:
Glycerol-free, lyophilization-compatible qPCR master mix, for extended room-temperature stability
Once lyophilized, millisecond rehydration times ideal for automated, high-throughput systems
Antibody-mediated hot-start DNA polymerase, for improved assay sensitivity and reliability
Optimized for fast, multiplex amplification of high- and low-copy targets

Storage and Stability:

 

 

Experimental Instructions:

Pre-lyophilization master mix preparation guidelines

 

Reagent

Volume

Final Concentration

 

2x SensiFAST Lyo-Ready No-ROX Mix

10 mL

1x

 

20 mM Forward Primer

0.4 mL

400 nM

 

20 mM Reverse Primer

0.4 mL

400 nM

 

10 μM Probe

0.2 mL

100 nM

 

Pre-Lyophilization Master Mix Volume

≤20 μL

 

 

qPCR mix setup guidelines
Rehydrate the lyophilized qPCR master mix in the reaction vials with 20 μL template-containing solution and run qPCR.

qPCR conditions

 

Cycles

Temp.

Time

Notes

 

1

95°C

2 min

Polymerase activation

 

40

95°C
60°C

10 sec
30 sec

 

Denaturation
Annealing/extension
(acquire at end of step)

 

 

 

Meet us at our next conference!

 

   

ASM Microbe

> Request a meeting

Booth:  3433

Dates:  7 - 11 June

Location:  Georgia World Congress Center, Atlanta, GA USA

 

Translational Research Symposium

> Request a meeting

Booth:  TBC

Dates:  26 July

Location:  Brisbane, QLD Australia

 

AACC 2018

> Request a meeting

Booth:  1080

Dates:  29 July - 2 Aug

Location:  McCormick Place, Chicago, IL USA

 

 

 

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