cGMP Floor Plan

C1 – C2 – C3 – C4 – C5 & 12 – C6 – C7 – C8 – C9 – C10 – C11,13 & 15– C14 – C16 & 17–

4°C refrigerator: Revco -20°C freezer -70°C freezer: So-Low Class 100 Downdraft Hood – Softwall (8'x12') HPLC Apparatus: Beckman, System Gold 126 Stainless steel tables Peristaltic pump: Bellco Chromatography columns

Clean Processed Glassware

Stainless steel table with HPLC computer and monitor RC-3B+ centrifuge and rotor: Sorvall RC-5B+ centrifuge and rotors (2): Sorvall Sink with Hot & Cold Water Pass through to room C13 Documentation bench Services: N2, Air, CO2, O2, vacuum

Chrome shelving for storage of glassware, pipettes, tubing

Position pointer over each room for more details

Class 100 Biological Safety Cabinet (6') Bellco Bioreactor - 36 liter (Capacity for 4 units) Controller, pumps and flow meters Peristaltic pump 27°C incubator: Precision Stir plates for 27°C incubator Inverted microscope

Protein Purification Area

Infected Cell Culture Lab

SS Microscope table Hemocytometer Water bath Microwave Coulter counter Services: N2, air, CO2, O2, vacuum

Class 100 Biological Safety Cabinet (6'): Nu-Air 35-37°C water bath Floor shaker/incubator: New Brunswick Stainless steel table Chrome shelves for storage Spectrophotometer: Spectronic 20 Genesys Sonicator: Fisher Trip balance Mechanical pipettor

Bacterial Culture Area

Mop Room

Shelves for storage of concentrated disinfectants Hooks (or other holders) for mops Mops and mop buckets

Uninfected Cell Culture Lab

Class 100 Biological Safety Cabinet (6') 4°C refrigerator Chrome storage shelves Peristaltic pump 27°C incubator: Precision

Stir plates for 27°C incubator Inverted microscope Microscope table HemocytometerWater bath 27°C Services: N2, Air, CO2, O2, vacuum

Formulations Area

Chemical Storage Area

Sink with WFI water Stainless steel table Balance pH meter Stir plate Dust/Exhaust hood Shelves for storage of supplies (weigh boats, spatulas, pH standards, graduated cylinders, beakers, etc.)

Chrome shelves for storage of chemicals Corrosives storage cabinet Flammables storage cabinet

Gowning Room

Storage racks and/or hooks for lab coats, etc. Caps Lab coats Alcohol Wipers Scissors Gloves Masks

Glassware Processing Area

Pharmaceutical Grade Autoclave: Steris Class 100 Pharmaceutical Oven: GruenbergSink with WFI water Shelf space for unprocessed glassware Hand washing sink : DeI Carts for transporting unprocessed glassware Carts for transporting processed glassware Solid waste receptacle

Hallway

Hand washing sink: DeI Gloves Storage space for gloves, masks, etc. Solid waste receptacles Carts

Hallway

Carts - 3 Booties Lint-free wipers Alcohol Shelving for storage of supplies Receptacle for lab coats (for laundry) Hooks for lab coats

Mechanical Access

Service access for autoclave Service access for oven Vacuum pump Air compressor Air dryer Purified water system (RO) Purified water system (DeI) Water heater

Water softener Carbon steel steam generator: Steris Pure steam generator: Steris WFI condenser: SterisWFI reservoir: Feldmeier Compressed gas manifolds and tanks (N2, O2, CO2)

Office

Hallway

C1 –
C2 –
C3 –
C4 –
C5 & 12 –
C6 –
C7 –
C8 –
C9 –
C10 –
C11,13 & 15–
C14 –
C16 & 17–

4°C refrigerator: Revco
-20°C freezer
-70°C freezer: So-Low
Class 100 Downdraft Hood –
Softwall (8'x12')
HPLC Apparatus: Beckman,
System Gold 126
Stainless steel tables
Peristaltic pump: Bellco
Chromatography columns

Stainless steel table with HPLC
computer and monitor
RC-3B+ centrifuge and rotor:
Sorvall
RC-5B+ centrifuge and rotors (2):
Sorvall
Sink with Hot & Cold Water
Pass through to room C13
Documentation bench
Services: N2, Air, CO2, O2, vacuum

Chrome shelving for storage
of glassware, pipettes, tubing

Class 100 Biological Safety
Cabinet (6')
Bellco Bioreactor - 36 liter
(Capacity for 4 units)
Controller, pumps and flow meters
Peristaltic pump
27°C incubator: Precision
Stir plates for 27°C incubator
Inverted microscope

SS Microscope table
Hemocytometer
Water bath
Microwave
Coulter counter
Services: N2, air, CO2, O2,
vacuum

Class 100 Biological Safety Cabinet (6'): Nu-Air
35-37°C water bath
Floor shaker/incubator: New Brunswick
Stainless steel table
Chrome shelves for storage
Spectrophotometer: Spectronic 20 Genesys
Sonicator: Fisher
Trip balance
Mechanical pipettor

Shelves for storage of concentrated disinfectants
Hooks (or other holders) for mops
Mops and mop buckets

Class 100 Biological Safety
Cabinet (6')
4°C refrigerator
Chrome storage shelves
Peristaltic pump
27°C incubator: Precision

Stir plates for 27°C incubator
Inverted microscope
Microscope table
Hemocytometer
Water bath 27°C
Services: N2, Air, CO2, O2,
vacuum

Sink with WFI water
Stainless steel table
Balance
pH meter
Stir plate
Dust/Exhaust hood
Shelves for storage of supplies
(weigh boats, spatulas, pH standards,
graduated cylinders, beakers, etc.)

Chrome shelves for storage of chemicals
Corrosives storage cabinet
Flammables storage cabinet

Storage racks and/or
hooks for lab coats, etc.
Caps
Lab coats
Alcohol
Wipers
Scissors
Gloves
Masks

Pharmaceutical Grade Autoclave: Steris
Class 100 Pharmaceutical Oven: Gruenberg
Sink with WFI water
Shelf space for unprocessed glassware
Hand washing sink : DeI
Carts for transporting unprocessed glassware
Carts for transporting processed glassware
Solid waste receptacle

Hand washing sink: DeI
Gloves
Storage space for gloves, masks, etc.
Solid waste receptacles
Carts

Carts - 3
Booties
Lint-free wipers
Alcohol
Shelving for storage of supplies
Receptacle for lab coats (for laundry)
Hooks for lab coats

Service access for autoclave
Service access for oven
Vacuum pump
Air compressor
Air dryer
Purified water system (RO)
Purified water system (DeI)
Water heater

Water softener
Carbon steel steam generator: Steris
Pure steam generator: Steris
WFI condenser: Steris
WFI reservoir: Feldmeier
Compressed gas manifolds and tanks (N2, O2, CO2)